Under Open Access philosophy, Redalyc aims to contribute to the editorial scientific activity produced in and about Ibero-America making available for public consultation the content of 550 scientific journals of different knowledge areas: http://redalyc.uaemex.mx
Hi!! Now I am growing T47D and I have experienced some of the problems that you described in your blog. In particular, I have notice that cells at high density (above 60%) show many vacuoles-like bodies (inclusion bodies). Is it normal? I am surprised that the picture you show from ATCC (right side) show many of them, which I have also observed in most of the dishes. Any suggestions is welcome. Contact at ggicazbalceta at gmail. Thanks!!!
By the way, I have also suspected about mycoplasma. Is it? The picture of high density from ATCC is very similar to that one I have seeing in my cultures when they become sick with mycoplasma even if the cells are growing almost well. But also I have observed that morphology when cells are growing well, very well.
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Who am I?
Last time my PI had to introduce me, he called me "a senior scientist". So I've moved beyond the post doc to... what? Let's see: an adjunct prof at PUI*, now a part-time prof at SLU**, and on top of it all, a mother of two. The future scientists that I'm training range in age from 4 to >24. It is often challenging, but never boring.
*Primarily undergraduate institution ** Small local univeristy.
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Sending an email to your gmail account now re: this issue
Under Open Access philosophy, Redalyc aims to contribute to the editorial scientific activity produced in and about Ibero-America making available for public consultation the content of 550 scientific journals of different knowledge areas: http://redalyc.uaemex.mx
I've been in that pickle more times than I can count. You'd think by now I'd have pdfs of my papers on all my computers, right? :)
chaussures converse Converse, la marque tendance
du moment. Quelque soit votre style, vous trouverez la converse à votre pied
Hi!! Now I am growing T47D and I have experienced some of the problems that you described in your blog. In particular, I have notice that cells at high density (above 60%) show many vacuoles-like bodies (inclusion bodies). Is it normal? I am surprised that the picture you show from ATCC (right side) show many of them, which I have also observed in most of the dishes. Any suggestions is welcome. Contact at ggicazbalceta at gmail. Thanks!!!
By the way, I have also suspected about mycoplasma. Is it? The picture of high density from ATCC is very similar to that one I have seeing in my cultures when they become sick with mycoplasma even if the cells are growing almost well. But also I have observed that morphology when cells are growing well, very well.
Thanks again!!
You can get the full print version. contact me at martinseo2010@hotmail.com
Yes, we need open access. I use it and amazing, I don't think that I'm the one who think that open access really help me.
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